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Quantitative Proteomics Center
Department of Biological Sciences
Graduate Course
A graduate course, Biol. 4260, Proteomics Laboratory has been established and will be available every fall semester.  This course provides an introduction to the field of proteomics and emphasizes hands-on experience with the MALDI-TOF, analysis of in-gel digests, and intensive experience in data analysis for both MALDI-TOF data and LC-MS fragmentation data. This course is open to both students in the M.A. in biotechnology and to all students in the Ph.D. programs.  This course will meet on Thursdays at 9:10 AM-12:15 PM.

Individual Training sessions:
Fee-based one-on-one training can be arranged for use of MALDI-TOF, in-gel digestion, and Mascot database searching.  Please Contact us for details.


Sample Preparation for DIGE Mini-Course
The preparation of samples for proteomics analysis is normally done by the collaborating laboratory. There are several reasons for this approach.  For example, cell cultures must be at the right stage of growth for the particular comparison and this will be under control of the collaborating laboratory.  The sample preparation mini-course provides an introduction to the technique of sample preparation for quantitative proteomics and provides detailed protocols for protein extraction, sample cleanup and protein solubilization.  Contact us to register for the next mini-course. Watch the news page for schedule.

Departmental and Lab Group Meetings
Does your lab group or department want to hear more about how quantitative discovery proteomics can enhance your research program? Contact us to schedule a talk for your group.

Lunchtime Proteomics Workshops
Learn ways to get more out of your proteomics and mass spectrometry efforts.  These informal workshops will provide examples of how to improve what you are already doing, and provide an introduction for newcomers to the field.

Topics planned include:
  • The central importance of calibration and methods for achieving better calibration
  • Automated data collection with the Voyager DE-Pro; methods and benefits
  • In-gel digestion for mass spectrometry - standard operating procedures and their importance
  • Database searching with Mascot: strategies for ensuring more accurate protein identifications
  • Automated data analysis with Mascot Daemon and other tools: methods and benefits
  • The 2D workshops:
                - The art and practice of isoelectric focusing
                - SDS PAGE in the 2D format - tricks and traps
  • Quantitative proteomics with the label-free shotgun technique - how it can strengthen your research program
  • Others?  Contact us

For schedules: watch the news page and department-wide notices.

Annual Open House
An open house is planned to introduce potential collaborators to the center and its capabilities.  Watch the news page for schedule.