A graduate course, Biol. 4260, Proteomics Laboratory
has been established and will be available every fall semester.
This course provides an introduction to the field of proteomics and
emphasizes hands-on experience with the MALDI-TOF, analysis of in-gel
digests, and intensive experience in data analysis for both MALDI-TOF
data and LC-MS fragmentation data. This course is open to both students
in the M.A. in biotechnology and to all students in the Ph.D.
programs. This course will meet on Thursdays at 9:10 AM-12:15 PM.
Individual Training sessions:
Fee-based one-on-one training can be arranged for use of MALDI-TOF,
in-gel digestion, and Mascot database searching. Please Contact us for details.
Sample Preparation for DIGE Mini-Course
The preparation of samples for proteomics analysis is normally done by
the collaborating laboratory. There are several reasons for this
approach. For example, cell cultures must be at the right stage
of growth for the particular comparison and this will be under control
of the collaborating laboratory. The sample preparation
mini-course provides an introduction to the technique of sample
preparation for quantitative proteomics and provides detailed protocols
for protein extraction, sample cleanup and protein
solubilization. Contact us to register for the next mini-course. Watch the news page for schedule.
Departmental and Lab Group Meetings
Does your lab group or department want to hear more about how
quantitative discovery proteomics can enhance your research program? Contact us to schedule a talk for your group.
Lunchtime Proteomics Workshops
Learn ways to get more out of your proteomics and mass spectrometry
efforts. These informal workshops will provide examples of how to
improve what you are already doing, and provide an introduction for
newcomers to the field.
Topics planned include:
The central importance of calibration and methods for achieving better calibration
Automated data collection with the Voyager DE-Pro; methods and benefits
In-gel digestion for mass spectrometry - standard operating procedures and their importance
Database searching with Mascot: strategies for ensuring more accurate protein identifications
Automated data analysis with Mascot Daemon and other tools: methods and benefits
The 2D workshops:
The art and practice of isoelectric focusing
SDS PAGE in the 2D format - tricks and traps
Quantitative proteomics with the label-free shotgun technique - how it can strengthen your research program